Last updated 11/12/2012
Authors: Vadym Sulimenko, Pavel Dráber
Institute of Molecular Genetics
Academy of Sciences of the Czech Republic, v. v. i.
Vídeňská 1083142 20 Prague 4, Czech Republic
Activation of mast cells is characterized by dramatic changes in cell morphology, intracellular transport and exocytosis. All these processes depend on the activities of cytoskeletal filamentous structures – microtubules, microfilaments and intermediate filaments. Different regulatory pathways regulate cytoskeletal functions indispensable for mast cell degranulation. Microtubules of activated mast cells has been studied in various cellular models ranging from mouse bone marrow-derived mast cells (BMMCs) and isolated rat peritoneal mast cells (RPMCs) to mouse BMMC lines (e.g. MC/9), rat basophilic leukemia cell lines (e.g. RBL-2H3) or human must cell lines (e.g. HMC-1).
Microtubules are cytoskeletal polymers essential for the maintenance of cell shape, division, migration, compartmentalization and ordered intracellular transport powered by motor proteins. Microtubule is a cylinder with outer diameter 25 nm formed by 13 laterally bound protofilaments built by heterodimers of globular α- and β-tubulin subunits arranged in a head-to-tail manner (1). Microtubules are polar structures with two structurally distinct ends – a slow-growing minus end, exposing α-tubulin subunits, and a fast-growing plus end exposing β-tubulin subunits (2). Within the cells minus ends are stabilized by anchoring in microtubule-organizing centers (MTOCs), whereas plus ends are highly dynamic and switch between phases of growth and shrinkage. Each tubulin monomer binds one molecule of GTP, nonexchangeable in α-subunit (N-site) and exchangeable in β-subunit (E-site). Polymerization and depolymerization of microtubules are driven by hydrolysis of GTP on β-tubulin monomer. GTP exchange is necessary for the switch between phases of growth and shrinkage separated by catastrophe (growth to shrinkage transition) and rescue (from shortening to growth). This dynamic instability is an essential feature of microtubules that allows them to search through the cell for targets like cell cortex or actin cytoskeleton (3). The dynamics of microtubules can be regulated by incorporation of alternative tubulin isotypes - eight α-tubulin and seven β-tubulin phylogenetically conserved genes were identified in human (4), by post-translational modifications of tubulin subunits (mainly acetylation, tyrosination/detyrosination, polyglutamylation, polyglycylation and phosphorylation), and binding of microtubule associated proteins MAPs (5, 6). Microtubule dynamics can be also regulated by a variety of ligands, including microtubule drugs, i.e. docetaxel (7). The key regulator of microtubule nucleation is the γ-tubulin (8), a highly conserved minor member of the tubulin family concentrated in MTOCs. Within the cells γ-tubulin interacts with 6 GCPs (γ-tubulin complex proteins) to create small (γ-TuSC) and large (γ-TuRC) complexes that participate in microtubule nucleation (9). The plus-end tracking proteins (+TIPs), a diverse group of molecules that display dynamic accumulation at the ends of growing microtubules, represent important regulators of microtubule plus end dynamics (10).
It is well established that microtubule drugs suppress exocytosis (11) and that movement of secretory granules in mast cells is dependent on intact microtubules (12-14). Activation of mast cells leads to changes in the topography of microtubules. FcεRI aggregation in BMMCs causes accumulation of microtubules in cell periphery (14, 15). Microtubules of BMMCs attached to fibronectin form protrusions in the course of mast cells activation; activation also modifies microtubule dynamics (16). The signaling pathway formed by tyrosine kinase Fyn, scaffolding adaptor protein Gab2 and Rho GTPase RhoA might play an important role in reorganization of microtubules after activation (14). It is well established that RhoA affects the dynamics of growing microtubules through its target mDia (17). Microtubule formation may also be influenced by +TIPs that interact at the cell periphery with cytoskeletal scaffold IQ domain GTPase-activating protein 1 (IQGAP1) which was found to sequester calmodulin and Rac GTPase (18). An important role in the regulation of microtubule formation and granule translocation in activated mast cells might also be played by RasGRPs that activate RhoA via PI3 kinase (19). Typical distribution of microtubules in resting and activated mouse BMMCs (16) is shown in the right panel.
The store-operated Ca2+ entry (SOCE) is important for the replenishment of intracellular Ca2+ stores in endoplasmic reticulum (ER). One key component of SOCE is protein STIM1 (stromal interacting protein 1), the Ca2+-sensor associated with ER (20). After Ca2+ depletion from ER stores, STIM1 accumulates into ER subregions in close proximity to plasma membrane (10-25 nm) (21). STIM1 contains the SxIP sequence responsible for its direct interactions with end binding protein 1 (EB1; +TIP) and association with microtubules (20). Comet-like movement of STIM1 is observed by live-cell imaging in resting mast cells (16, 22). This movement is substantially reduced after mast cell activation (23, 24). Although microtubules could play a supportive role in SOCE signaling, several lines of evidence indicate that interaction of STIM1 with EB1 is not essential for the transport of STIM1 to plasma membrane (16).
Formation of microtubules is effectively regulated by microtubule nucleation. After mast cell activation, the Src-family kinases Lyn and Fyn form complexes containing γ-tubulin and proteins phosphorylated on tyrosine (15, 25). Src family kinases in mast cells form an essential part of signal transduction pathways located near the plasma membrane. However, they were also found in MTOC where they might regulate centrosomal recruitment of γ-tubulin (9). The Syk kinase, another important component of signaling pathways in activated mast cells, is capable of phosporylating α-tubulin (26) and interacts with γ-tubulin (15). Tyrosine kinases can thus play an important role in modulation of microtubules in activated mast cells (15, 27).
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